Function and Structure in Ribonucleic Acid Phage Qβ Ribonucleic Acid Replicase
نویسندگان
چکیده
منابع مشابه
Function and structure in ribonucleic acid phage Q beta ribonucleic acid replicase. The roles of the different subunits in transcription of synthetic templates.
Phage Q/3 RNA-dependent RNA replicate consists of four subunits: one is RNA phage specific (subunit II), the other three, present in the uninfected cell, are the protein biosynthesis elongation factors EF-Tu (subunit III) and EF-Ts (subunit IV) and the translation interference factor i (subunit I). When the protein biosynthesis elongation factors are removed by centrifugation of the initiated e...
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The formation and some properties of a stable complex between valyl transfer ribonucleic acid synthetase and transfer RNA from yeast have been investigated by three different methods for the isolation of the complex: titration over Sephadex, sucrose gradient centrifugation, and electrophoresis on Pevikon as supporting medium. Transfer RNA specific for valine (tRNAvsl) can be separated into two ...
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The formation of a stable complex between valyl transfer ribonucleic acid synthetase from yeast and transfer RNA specific for valine (tRNAVa’) from the same source has been used as a model for the recognition between enzyme and tRNA. With the use of sucrose gradient centrifugation to isolate the complex, the major fraction of tRNAVal (tRNAp’) was found to retain its ability to form a stable com...
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The mechanism of the biosynthesis of deoxyribose has been one of the particularly obscure problems in the biochemistry of the nucleic acids. During the past decade, considerable effort was expended in the demonstration that the deoxyribose of deoxyribonucleic acid was not derived via the condensation of glyceraldehyde a-phosphate and acetaldehyde in the presence of deoxyribose phosphate aldolss...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79888-1